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KMID : 0380020140290040232
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Korean Journal of Biotechnology and Bioengineering
2014 Volume.29 No. 4 p.232 ~ p.238
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Simultaneous Determination of Creatine, Dicyandiamide and Dihydrotriazine in Dietary Supplements by High Performance Liquid Chromatography
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Park Sang-Wook
Yoo Myung-Sang
Lee Won-Jae
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Abstract
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The simultaneous determination of creatine monohydrate (CrM), dicyandiamide and dihydrotriazine in dietary supplements using reversed-phase high performance liquid chromatography (HPLC) was developed. Chromatography was performed on a Nuclosil 100-5 SA (4.6×250 mm) column with a mobile phase of 2.3% ammonium phosphate (pH 5.5), and UV detection at 224 nm, 212 nm, and 237 nm, respectively. The performance characteristics of HPLC were determined in terms of selectivity, linearity, precision, recovery, limit of detection (LOD), and limit of quantification (LOQ). The calibration curves were linear within the concentration range of 40.0 ~500.0 μg/mL for creatine, 0.1~12.8 μg/mL for dicyandiamide, and 0.05~6.4 μg/mL for dihydrotriazine. The detection limits of the method were 1.09, 0.01, and 0.08 μg/mL for creatine, dicyandiamide, and dihydrotriazine, respectively. The recoveries of creatine, dicyandiamide, and dihydrotriazine were 97.2~100.9, 92.3~106.5, and 97.2~105.5%, respectively. It is expected that the chromatographic analytical method developed in this study will be usefully applicable to simultaneous determination of creatine, dicyandiamide, and dihydrotriazine contained in dietary supplements.
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KEYWORD
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Creatine, Dicyandiamide, Dihydrotriazine, High performance liquid chromatography, Simultaneous determination
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